Antiviral effect of lamivudine on HIV-1 targeting MT2 cells influenced by morphine
10.3760/cma.j.issn.0254-6450.2011.07.016
- VernacularTitle:吗啡对拉米夫定抗HIV-1药效影响的体外MT2细胞实验研究
- Author:
Bing-Yu LIANG
1
;
Dao-Min ZHUANG
;
Jun-Jun JIANG
;
Si-Yang LIU
;
Qi-Jian SU
;
Jing-Yun LI
;
Hao LIANG
Author Information
1. 广西医科大学
- Keywords:
HIV-1;
Morphine;
Lamivudine;
Inhibition
- From:
Chinese Journal of Epidemiology
2011;32(7):705-708
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine whether morphine having the ability to influence the antiviral effect of lamivudine(3TC)in vitro study.Methods MT2 cells were randomly assigned into morphine+3TC treatment group,morphine+naloxone+3TC treatment group,naloxone+3TC treatment group.Both 3TC and virus control groups were set up.The corresponding MT2 cells were treated with opiates antagonist(naloxone)for 0.5 hours before the 24-hours morphine treatment program was implemented while all of the groups were then infected with equal amounts of cell-free HIV-1 ⅢB strain and 3TC.HIV-1 p24 antigen in culture supernatants collected at days 3,4,5 and 6after infection status was tested and the inhibition of 3TC anti-HIV-1 p24 antigen of various treatment groups calculated.Results Inhibition of 3TC anti-HIV-1 p24 antigen of Morphine+3TC treatment group was the lowest when HIV-1 infected cells at 3rd and 4th day and showed significant difierence (P<0.05)when compared to the 3TC control.However,there was no statistically significant difference among them(P>0.05),when virus was infected the cells at 5th and 6th day.The difference of 3TC anti-HIV-1 p24 antigen inhibition between the morphine+naloxone+3TC treatment group and the naloxone+3TC treatment group was not significant(P>0.05).Similar results were obtained when these two groups were compared to the 3TC control group(P>0.05),respectively.The 3TC anti-HIV-1 p24 antigen inhibition of each treatment group reduced as the time of infection prolonged,showing a significant and time-course effbct.Conclusion The 3TC antiviral effect was reduced by morphine in the early stage of infection,and could be blocked by naloxone.
