The role of MDR1 gene in volume-activated chloride currents in pigmented ciliary epithelial cells.
- Author:
Li-Xin CHEN
1
;
Li-Wei WANG
;
Tim JACOB
Author Information
1. Laboratory of Cell Biology, Guangdong Medical College, Zhanjiang 524023, China Cardiff School of Biosciences, Cardiff University, Cardiff, UK. CHENL1@cardiff.ac.uk
- Publication Type:Journal Article
- MeSH:
ATP-Binding Cassette, Sub-Family B, Member 1;
biosynthesis;
Animals;
Cattle;
Cells, Cultured;
Chloride Channels;
physiology;
Ciliary Body;
cytology;
physiology;
Epithelial Cells;
metabolism;
physiology;
Gene Expression;
drug effects;
Genes, MDR;
physiology;
Oligonucleotides, Antisense;
pharmacology
- From:
Acta Physiologica Sinica
2002;54(1):1-6
- CountryChina
- Language:English
-
Abstract:
The role of multidrug resistance (MDR1) gene in the activation of volume-activated chloride currents in bovine pigmented ciliary epithelial (PCE) cells was investigated by the patch-clamp technique, the antisense approach, the immunofluorescent technique and the confocal microscopy. PCE cells express P-glycoprotein (P-gp, the product of MDR1 gene). An MDR1 antisense oligonucleotide suppressed MDR1 expression (93% reduction of P-gp immunofluorescence), delayed the activation of a volume-activated chloride current (latency prolonged by 109%), reduced the activation rate by 62% and decreased the peak value of the current by 56%. The transfection reagent lipofectin and the mismatch control oligonucleotide did not significantly affect the current. The data indicate that the volume-activated chloride current is associated with the endogenous expression of MDR1 gene in PCE cells.
