Interleukin-1beta expression and phospholipase A(2) activation after intestinal ischemia/reperfusion injury.
- Author:
Guang-Tao YAN
1
;
Xiu-Hua HAO
;
Hui XUE
;
Lu-Huan WANG
;
Ying-Li LI
;
Li-Ping SHI
Author Information
1. Laboratory of Biochemistry, Basic Medicine Institute, General Hospital of PLA, Beijing 100853, yan301@263.net
- Publication Type:Journal Article
- MeSH:
Animals;
Female;
Gene Expression;
Interleukin-1;
biosynthesis;
metabolism;
Intestines;
blood supply;
Ischemia;
metabolism;
Male;
Phospholipases A;
biosynthesis;
metabolism;
RNA, Messenger;
biosynthesis;
Rats;
Rats, Wistar;
Reperfusion Injury;
metabolism
- From:
Acta Physiologica Sinica
2002;54(1):28-32
- CountryChina
- Language:English
-
Abstract:
The experiments were carried out to explore the interactions between IL-1 beta gene expression, protein level and phospholipase A(2) PLA(2) inhibition after intestinal ischemia/reperfusion injury. Using a rat intestinal ischemia/reperfusion injury model, after collecting the serum, lung lavage, abdomen cavity lavage and important organ tissue samples from control, injury and PLA(2) inhibitor treated groups, IL-1 beta level was measured by radioimmunoassay, and the mRNA expression of IL-1 beta and type II PLA (2)was determined by RT-PCR. After 6 h of injury, the IL-1 beta level in serum was significantly higher than that in the control group; an increase in IL-1 beta was also observed in abdomen cavity lavage 1 or 3 h after injury. IL-1 beta was significantly increased in liver tissue after injury, but was not changed obviously in the lung, kidney and intestinal tissues. IL-1 beta in the lung lavage was significantly higher than that of control group. The mRNA expression of IL-1 beta in lung tissue was increased after injury, but type II PLA(2) mRNA expression was decreased. There were different changes in IL-1 beta level and gene expression after treatment with PLA(2) inhibitor chloroquine, cyclo-oxidase inhibitor indomethacin, or PAF receptor antagonist SR27417 respectively after injury. All these results indicate that after intestinal ischemia/reperfusion injury, the IL-1 beta level and mRNA gene expression are significantly increased, however, the relationship among IL-1 beta, PLA(2) activation and its metabolite release remains to be further elucidated.
