Recombinant human interleukin-10 inhibits vascular smooth muscle cell proliferation induced by TNF-alpha.
- Author:
Ping OUYANG
1
,
2
,
3
;
Li-Sheng PENG
;
Hong YANG
;
Wen-Yan WU
;
An-Long XU
Author Information
1. College of Life Science, Zhong Shan University, Guangzhou 510275
2. ls36@zsu.edu.cn
3. ls36@zsu.edu.cn
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Cycle;
drug effects;
Cell Division;
drug effects;
Cells, Cultured;
Interleukin-10;
pharmacology;
Male;
Mitogen-Activated Protein Kinases;
biosynthesis;
Muscle, Smooth, Vascular;
cytology;
drug effects;
enzymology;
Rats;
Rats, Sprague-Dawley;
Recombinant Proteins;
pharmacology;
Tumor Necrosis Factor-alpha;
pharmacology
- From:
Acta Physiologica Sinica
2002;54(1):79-82
- CountryChina
- Language:Chinese
-
Abstract:
Vessel injury provokes a release in proinflammatory cytokines that influence vascular smooth muscle cell (VSMC) proliferation. The purposes of this study was to determine the effects of recombinant human interleukin-10 (rhIL-10) on rat vascular smooth muscle cell proliferation and the activity of p44/p42 mitogen-activated protein kinase (MAPK) promoted by tumor necrosis factor-alpha (TNF-alpha). Rat aortic VSMCs were cultured and treated with rhIL-10 or TNF-alpha respectively, and then cotreated with rhIL-10 and TNF-alpha. The proliferation of VSMCs was quantified by colormetric assay. Cell cycle analysis was performed by flow cytometry. The p44/42 MAPK activity was evaluated by the immunoblotting technique using anti-p44/42 phospho-MAPK antibody. Compared to control group, TNF-alpha stimulated significantly VSMC proliferation in TNF-alpha group. rhIL-10 alone had no effect on VSMC growth, but significantly inhibited VSMC proliferation induced by TNF-alpha at a dose of 10 ng/ml. The cell number in G(0)/G(1) phase of TNF-alpha and rhIL-10 co-treatment group was higher than that of TNF- alpha group (P<0.01) by flow cytometry analysis. The p44/42 MAPK activity was significantly enhanced by TNF-alpha and the TNF-alpha effect was opposed by rhIL-10. It is suggested that rhIL-10 can inhibit TNF-alpha induced VSMC proliferation and phosphorylation of p44/42 MAPK.
