Studies on callus growth and phillyrin accumulation of Forsythia suspensa.
- Author:
You-qi LIU
1
;
Xing-chun TANG
;
De-tian CAI
Author Information
- Publication Type:Journal Article
- MeSH: Culture Media; Culture Techniques; Forsythia; chemistry; cytology; metabolism; Glucosides; biosynthesis; Lighting; Plant Growth Regulators; pharmacology; Plants, Medicinal; chemistry; cytology; metabolism
- From: China Journal of Chinese Materia Medica 2003;28(4):321-323
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of physical and chemical factors on callus growth and phillyrin contents of F. suspensa.
METHODThe cell growth index and phyllirin yield in different culture condition such as different plant hormones mixed, mediums, light and dark were compared. HPLC was used to examine phillyrin contents.
RESULT AND CONCLUSIONGrowth cycle of cells is twenty-eight days. During the course of callus growth, the processes of phillyrin biosynthesis were parallel with the cell growth. The optimum medium is MS. The optimum hormones concentrations are 1 mg.L-1 2,4-D, 0.5 mg.L-1 6-BA and 0.5 mg.L-1KT. The cell culture in light is more suitable than that in dark.
