Study on preparation process and analytical methods of ESAC from Ganoderma lucidum.
- Author:
Shu-ming HUANG
1
;
Xin-lin YANG
;
Bang-wu WANG
;
He-sun ZHU
;
Jian-lan XU
Author Information
- Publication Type:Journal Article
- MeSH: Drugs, Chinese Herbal; analysis; isolation & purification; Ethanol; Reishi; chemistry; Technology, Pharmaceutical; methods; Triterpenes; analysis; isolation & purification
- From: China Journal of Chinese Materia Medica 2003;28(4):332-334
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop the procedure for separating the ethanol-soluble and acidic components (ESAC) from Ganoderma lucidum, and to establish a method for quantifying ESAC in G. lucidum.
METHODThe ethanol extract of G. lucidum was extracted with a saturated NaHCO3 solution, acidified and re-extracted by chloroform to obtain ESAC. The quantitative analysis of ESAC was based on the characteristic color reaction between ESAC and H2SO4.
RESULTThe optimal conditions for separating ESAC on a 10 g scale are as follows: ratio of material and ethanol (mL), 1:15; immersing time, 24 h; volume of saturated NaHCO3 and chloroform, 1300 mL; extract 3 times. The condition for measuring ESAC is as follows: sample weight, 1 g; solution volume, 1.5 mL; immuersing time, 0.5 h; detecting reagent, 50% H2SO4 in ethanol; heating time in 100 degrees C water bathe, 3 min; measuring wavelength, 490 nm.
CONCLUSIONThe procedure for ESAC preparation is simple and well-designed, and the established method for ESAC can be used for the qualitative analysis of the G. lucidum related products.
