Determination of aloin in aloes by HPLC.
- Author:
Hong CAO
1
;
Yun LIU
Author Information
- Publication Type:Journal Article
- MeSH: Aloe; chemistry; Chromatography, High Pressure Liquid; methods; Emodin; analogs & derivatives; analysis; Plants, Medicinal; chemistry
- From: China Journal of Chinese Materia Medica 2003;28(4):349-351
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo describe a simple and rapid isocratic reversed-phase high performance liquid chromatography method for the baseline separation, identification and assay of aloin in aloes.
METHODThe analytical column was a ZORBAX SB-C18(4.6 mm x 250 mm) filled with a 5 microns stationary phase. The mobile phase consisted of acetonitrile-water (25:75); the flow-rate was 1 mL.min-1. The injection volume was 10 microL. The DAD detector was set at 355 nm.
RESULTThe calibration curve was linear over the range of 0.17-5.9 micrograms (r = 0.9999). The average recovery of the method was 98.6%, RSD 1.32% (n = 6).
CONCLUSIONThe results showed that this method was reliable and accurate. The method was applied to eleven Cape and East African aloes of different origin.
