The study of yi-shen-huo-xue fang's effects on expression of GMP-140 and cleaning out the oxygenic free radicle on rabbits blood stasis model.
- Author:
Xiao-ping ZHAN
1
;
Qing FANG
;
Jian-guo LOU
;
Xi-hong XU
;
Xiao-ying JIN
;
Guang-ming QIN
;
Jun BAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Drug Combinations; Drugs, Chinese Herbal; isolation & purification; pharmacology; Erythrocytes; metabolism; Female; Free Radical Scavengers; pharmacology; Lipid Peroxides; blood; Male; Medicine, Chinese Traditional; P-Selectin; biosynthesis; Plants, Medicinal; chemistry; Rabbits; Random Allocation; Superoxide Dismutase; blood
- From: China Journal of Chinese Materia Medica 2003;28(4):355-358
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effects of Yi-Shen-Huo-Xue Fang on expression of GMP-140 and cleaning out the oxygenic free radicle on rabbits blood stasis model.
METHODThirty rabbits were divided randomly into five groups as the normal group, model group, large dose of "Yi-Shen-Huo-Xue Fang" group, small dose of "Yi-Shen-Huo-Xue Fang" group and "Xue-Shuan-Xin-Mai-Ning" group. After being treated respectively, granule membrane protein 140(GMP-140), erythrocyte sueroxide dismutase (E-SOD), erythrocyte lipid peroxide(E-LPO), plasma lipid peroxide(P-LPO) were checked up.
RESULTThe GMP-140, E-SOD, E-LPO, P-LPO in normal control were compared with those in model groups, With the difference(P < 0.01), model control group was compared with large dose group and small dose group (P < 0.01), with "Xue-Shuan-Xin-Mai-Ning" group(P < 0.05), large dose group was compared with "Xue-Shuan-Xin-Mai-Ning" group(P < 0.05), and large dose group were compared with small dose group (P > 0.05).
CONCLUSIONThe model was made successfully. Large dose group, small dose group and "xue-shuan-xin-mai-ning" group can inhibit expression of GMP-140, enhence SOD activity and decrease LPO content on blood stasis rabbit model. Large dose group and small dose group have stronger effect than "xue-shuan-xin-mai-ning" group.
