Effects of varglaucocalyx on c-fos gene expression during global myocardial ischemia-reperfusion in rat.
- Author:
Lan-di LIU
1
;
Li-ka YE
;
Dong-jun PAN
;
Yan-duo JIANG
;
Tie-zheng ZHANG
;
Hui YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cardiotonic Agents; pharmacology; Drugs, Chinese Herbal; isolation & purification; pharmacology; Female; Gene Expression Regulation; drug effects; Genes, fos; Isodon; chemistry; Male; Myocardial Reperfusion Injury; etiology; metabolism; Myocardium; metabolism; Plants, Medicinal; chemistry; Proto-Oncogene Proteins c-fos; biosynthesis; Random Allocation; Rats; Rats, Wistar
- From: China Journal of Chinese Materia Medica 2003;28(4):358-361
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the effects of Varglaucocalyx on c-fos gene expression during global myocardial ischemia-reperfusion.
METHODForty Wistar rats were divided into 5 groups: group N as control; group CN as ischemia-reperfusion control and group XH, XM and XL treated with Varglaucocalyx 5%, 1%, 0.5% respectively prior to ischemia-reperfusion. The isolated rat hearts were perfused in condition of constant temperature and pressure, and then the left ventricular myocardiums were extracted for use. The expression of c-fos protein was detected by immunochemical method. The expression of c-fos protein were quantified by using computer image analysis system.
RESULTCompared with the values of group N, protein expressions relative area of c-fos gene (PERA) were increased significantly in group CN, XH, XM, XL(P < 0.01), but decreased significantly in group XH, XM, XL, compared with those of group CN (P < 0.05). The PERA of c-fos gene in group XM, XL were significantly lower than in group XH (P < 0.01), and the PERA of c-fos gene in group XM were lower than in group XL(P < 0.05).
CONCLUSIONVarglaucocalyx can effectively depress the expression of c-fos gene in myocardium which may account for its protection against myocardial ischemia-reperfusion injury, and the middle and the low concentrations of Varglaucocalyx are more effective than the high concentrations.
