Investigation on inhibitory and apoptosis-inducing effects of saponins from Tribulus terrestris on hepatoma cell line BEL-7402.
- Author:
Bin SUN
1
;
Wei-jing QU
;
Xiao-ling ZHANG
;
Huang-jian YANG
;
Xiu-yuan ZHUANG
;
Ping ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Cell Line, Tumor; Cell Proliferation; drug effects; Drugs, Chinese Herbal; isolation & purification; pharmacology; Humans; Liver Neoplasms; metabolism; pathology; Plants, Medicinal; chemistry; Proto-Oncogene Proteins c-bcl-2; metabolism; Saponins; isolation & purification; pharmacology; Tribulus; chemistry
- From: China Journal of Chinese Materia Medica 2004;29(7):681-684
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the inhibitory and apoptosis-inducing effects of saponins from Tribulus terrestris (STT) on liver cancer cell line BEL-7402.
METHODMTT, SRB, Wright staining, acridine orange staining, flow cytometry, and Immunofluorescence microscopy were used to evaluate the effects of STT on BEL-7402 cell line.
RESULTSMT had potent inhibitory effect on BEL-7402 cell line in a concentration-dependent manner. BEL-7402 cells exibited typical morphological alteration of apoptosis when sub-G1 peak could be seen. The expression of Bcl-2 was decreased in STT treated cells as compared with untreated control cells.
CONCLUSIONSTT exerts its cytotoxic effect on BEL-7402 cells by inducing apoptosis.
