Effect of Tujian decoction on protein kinase C activity in renal cortex in diabetic rat.
- Author:
Yuan-lin PIAO
1
;
Xiao-chun LIANG
;
Nan YANG
;
De-hai YIN
;
Ping-ping ZUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cuscuta; chemistry; Diabetes Mellitus, Experimental; enzymology; pathology; Drug Combinations; Drugs, Chinese Herbal; isolation & purification; pharmacology; Euonymus; chemistry; Kidney Cortex; enzymology; Kidney Glomerulus; pathology; Male; Plants, Medicinal; chemistry; Protective Agents; pharmacology; Protein Kinase C; metabolism; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2004;29(7):685-689
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate influence of administration of Tujian decoction (Chinese herbal medicine) on protein kinase C (PKC) activity, renal function and structure in diabetic rat kidney.
METHODExperimental diabetic nephropathy model was induced by nephrectomy combined with streptozotocin (STZ) injection in sprague-dawley rat. Tujian decoction (20 g x kg(-1) x d(-1)) and Valsartan (20 mg x kg(-1) x d(-1)) were orally administrated respectively for 12 weeks. PKC activity was measured by [3H]phorbol 12, 13-dibutyrate ([3H]PDBu) binding assay. 24 h urine protein excretion (Upro) and renal pathological changes were observed.
RESULTIn 12th week, diabetic nephrectomized rats developed proteinuria, glomerulosclerosis, increased membrane PKC activity (mPKC), decreased cytosol PKC (cPKC), and increased ratio of mPKC and cPKC (M/C). Administration of Tujian decoction or Valsartan led to a reduction in proteinuria, structural injury, mPKC and M/C, and a recovery in cPKC.
CONCLUSIONTujian decoction possesses a renoprotective effect on diabetic nephrectomized rat, at least partially via the inhibition of PKC activation in renal cortex.
