Study on genetic diversity of herba Cistanches by RAPD.
- Author:
Guang-Hong CUI
1
;
Min CHEN
;
Lu-Qi HUANG
;
Su-Ping XIAO
;
Da LI
Author Information
- Publication Type:Journal Article
- MeSH: Cistanche; classification; genetics; DNA, Plant; genetics; Genetic Variation; Genetics, Population; Plants, Medicinal; genetics; Polymorphism, Genetic; Random Amplified Polymorphic DNA Technique
- From: China Journal of Chinese Materia Medica 2004;29(8):727-730
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the genetic diversity of Cistanche species.
METHODTwo populations of Cistanche deserticola and four populations of C. tubulosa were analyzed by random amplified polymorphic DNA (RAPD) markers.
RESULTA total of 76 and 87 loci were amplified using 10 random primers each other. The average percentage of polymorphic loci of C. deserticola was 47.37%. The PPL were 39.47% and 35.53% for two populations. Average Nei's gene diversity was 0.1358, Shannon' s genetic diversity was 0.2072, and Gst was 0.2546. The average PPL of C. tubulosa was 27.59%. It was 19.54% to 25.29% in different populations and Andi'er population had the highest. Average Nei's gene diversity was 0.0823, and Shannon' s genetic diversity was 0.125 8, Cst was 0.175 5.
CONCLUSIONThe diversity of Cistanche deserticola is higher than that of C. tubulosa, but both has differentiation among populations, C. deserticola has already separated itself into two different ecotypes.
