Quality evaluation of prepared slices of Paeonia lactiflon--determination of paeoniflorin by HPLC.
- Author:
Xiao-Mei XIE
1
;
Chang-Zhu YU
;
Heng XU
;
Sheng WANG
;
Dian-Lei WANG
;
Ling ZHANG
;
Gui-Xin CHOU
;
Zheng-Tao WANG
Author Information
- Publication Type:Journal Article
- MeSH: Benzoates; analysis; Bridged-Ring Compounds; analysis; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; Glucosides; analysis; Monoterpenes; Paeonia; chemistry; Plants, Medicinal; chemistry; Quality Control; Technology, Pharmaceutical
- From: China Journal of Chinese Materia Medica 2004;29(8):759-762
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the quality criteria of the prepared slices of Paeonia lactiflon.
METHODRP-HPLC was used for the determination of paeoniflorin in 10 lots of samples by ultrasound-assisted extraction.
RESULTThe samples were extracted with 50% methanol. Seperation of the solution was performed on an ODS column with a mobile phase of acetonitrile-water (18:82), detected at 230 nm.
CONCLUSIONThe method is simple, repeatable, accurate and applicable.