Quantification of isopimpinellin in root of Toddalia asiatica by HPLC.
- Author:
Xiao-Yan HAO
1
;
Xiao-Hong CAO
;
Yan LIANG
;
Yue-Mao SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Furocoumarins; analysis; Plant Roots; chemistry; Plants, Medicinal; chemistry; Rutaceae; chemistry
- From: China Journal of Chinese Materia Medica 2004;29(8):768-769
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the content of isopimpinellin in root of Toddalia asiatica.
METHODSA HPLC method was set up. Using Hypersil C18 column and methanol-water (70:30) as mobile phase, with the detection wavelength at 306 nm.
RESULTThe linear range of isopimpinellin was 0.004 20 approximately 0.420 microg. The average recovery was 99.7% and the RSD 2.8%.
CONCLUSIONThe method is simple and accurate, with good reproducibility, and can be used as a quantitative analysis method for isopimpinellin.
