Effects of puerarin on number and activity of endothelial progenitor cells from peripheral blood.
- Author:
Fu-Rong ZHANG
1
;
Jun-Zhu CHEN
;
Jun-Hui ZHU
;
Xing-Xiang WANG
;
Jian-Hua ZHU
;
Yun-Peng SHANG
;
Xiao-Gang GUO
;
He-Min DAI
;
Jian SUN
Author Information
- Publication Type:Journal Article
- MeSH: Cell Adhesion; drug effects; Cell Division; drug effects; Cell Movement; drug effects; Cells, Cultured; Endothelial Cells; cytology; drug effects; Humans; Isoflavones; isolation & purification; pharmacology; Neovascularization, Physiologic; drug effects; Plants, Medicinal; chemistry; Pueraria; chemistry; Stem Cells; cytology; drug effects; Time Factors; Veins; cytology
- From: China Journal of Chinese Materia Medica 2004;29(8):777-781
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate whether puerarin can augment endothelial progenitor cells (EPCs) numbers, promote EPC proliferation, migration and adhesion.
METHODTotal mononuclear cells (MNCs) were isolated from peripheral blood by Ficoll density gradient centrifugation, and then the cells were plated on fibronectin-coated culture dishes. After 7 days culture, attached cells were stimulated with puerarin (to make a series of final concentrations: 0. 1, 0.5, 1, 3 mmol x L(-1)) or vehicle control for the respective time points (6, 12, 24, 48 h). EPCs were characterized as adherent cells double positive for DiLDL-uptake and lectin binding by direct fluorescent staining under a laser scanning confocal microscope. EPCs proliferation, migration and in vitro vasculogenesis activity were assayed with MT assay, modified Boyden chamber assay and in vitro vasculogenesis kit, respectively. EPCs adhesion assay was performed by replating those on fibronectin-coated dishes, then adherent cells were counted.
RESULTIncubation of isolated human MNCs with puerarin dose increased the number of EPCs, maximum at 3 mmol x L(-1), 24 hours (approximately 1-fold increase, P < 0.01). In addition, puerarin also promoted EPC proliferative, migratory, adhesive and in vitro vasculogenesis capacity.
CONCLUSIONPuerarin can augment the number of EPCs with enhanced functional activity.