Selection of sodium chloride tolerant mutants in Chrysanthemum morifolium in Anhui.
- Author:
Jian-ping XUE
1
;
Ai-ming ZHANG
;
Xiang GAO
;
Wei SHENG
Author Information
- Publication Type:Journal Article
- MeSH: Chrysanthemum; drug effects; genetics; growth & development; Culture Media; Culture Techniques; methods; Mutation; Plant Leaves; growth & development; Plants, Medicinal; drug effects; genetics; growth & development; Sodium Chloride; pharmacology
- From: China Journal of Chinese Materia Medica 2004;29(9):834-837
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo select the sodium chloride tolerant mutants of the Chrysanthemum morifolium callus through tissue culture and EMS (ethylmethane sulfonate) treatment.
METHODCalli were induced from the leaves of C. morifolium. The calli were treated with 0.2% and 0.5% EMS, respectively. After 15 days' culture, the calli were transplanted to selection media with 0.5%, 1.0% and 1.5% NaCl, and the sodium chloride tolerant mutants were selected out.
RESULT AND CONCLUSIONAfter tested the sodium chloride tolerant stability, the callus selected are found to be the mutants indeed.
