Determination of gentiopicroside in different morphological types of cultivated Gentiana manshurica population.
- Author:
Chen WANG
1
;
Xiu-fang XING
;
Li-xiang MA
;
Yang GUAN
;
Shao-jun DAI
;
Ming-yuan LIU
Author Information
- Publication Type:Journal Article
- MeSH: Genetic Variation; Gentiana; anatomy & histology; chemistry; classification; Glucosides; analysis; Iridoid Glucosides; Iridoids; analysis; Plant Roots; anatomy & histology; chemistry; Plants, Medicinal; anatomy & histology; chemistry; classification; Pyrans; analysis; Species Specificity
- From: China Journal of Chinese Materia Medica 2004;29(9):841-844
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the correlation between morphological variation and gentiopicroside content in cultivated Gentiana manshurica roots and to investigate the feasibility of breeding new varieties of high effective constituent content.
METHODGentiopicroside was determined in 5 morphological types of cultivated G. manshurica roots by HPLC, which are normal (or wild) type, white-flowered type, thick-rooted type, broad-leaved type I and broad-leaved type II.
RESULTAmong different types gentiopicroside content is the highest in the roots of thick-rooted type, the contents decrease as following order: normal type, broad-leaved type I white-flowered type and broad-leaved type II, and the gentiopicroside contents in the same type root system are a positive correlation with root ages, as 3-years-age roots > 2-years-age roots > 1-year-age varied with roots.
CONCLUSIONThe contents of effective constituent vary with the morphological variation in cultivated G. manshurica. It is feasible to breed a new variety with high effective constituent with the morphological character as a selecting index.
