Determination of luteolin-7-O-glycoside in the herb of Dracocephalum rupestra by HPLC.
- Author:
Dong-mei REN
1
;
Hong-xiang LOU
;
Bin MA
;
Mei JI
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Glycosides; analysis; Lamiaceae; chemistry; Luteolin; analysis; Plant Leaves; chemistry; Plant Stems; chemistry; Plants, Medicinal; chemistry; Seasons
- From: China Journal of Chinese Materia Medica 2004;29(9):860-862
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a method for determination of luteolin-7-O-glycoside in the roots, stems, leafs, flowers and aerial parts of Dracocephalum rupestra sampled in different seasons.
METHODThe samples were analyzed on an phenomenex C18 column, with mobile phase of methanol-acetonitrile-0.4% phosphoric acid (30:8:62) at flow rate 1.0 mL x min(-1) and detection at wavelength of 350 nm.
RESULTThe content of luteolin-7-O-glycoside in different parts of D. rupestra was different maximum in leaves, while minimum in stems. Luteolin-7-O-glycoside in D. rupestra sampled before blossoming was the highest.
CONCLUSIONThe method simple, accurate and suitable for the quality evaluation of this plant medicine.
