Effect of ginsenoside Rg1 on the influence of neprilysin expression induced by LPS in BT325 cell line.
- Author:
Huan-min LUO
1
;
Hui DENG
;
Feng HUANG
;
Fei XIAO
;
Xiao-guang LI
;
Pei-fen ZHANG
;
Qin GAO
;
Wen WENG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cell Survival; drug effects; Drugs, Chinese Herbal; pharmacology; Ginsenosides; isolation & purification; pharmacology; Glioma; metabolism; pathology; Humans; Lipopolysaccharides; Neprilysin; metabolism; Neuroprotective Agents; pharmacology; Panax; chemistry; Plants, Medicinal; chemistry
- From: China Journal of Chinese Materia Medica 2004;29(9):890-893
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of ginsenoside Rg1 on the influence of neprilysin expression induced by LPS in BT325 cell line.
METHODMTT was used to measure the levels of the survival rate of BT325 cultured with ginsenoside Rg1 and LPS in different concentrations. The expression of NEP was measured by RT-PCR.
RESULTThe survival rate of BT325 was obviously inhibited by LPS, and the expression of NEP was decreased. The survival rate of BT325 was obviously raised by ginsenoside Rg1, and the expression of NEP was increased.
CONCLUSIONLPS can cause cell damage, and decrease the expression of NEP. Ginsenoside Rg1 can exert neuroprotective properties which protects BT325 cell line from the cell toxicity of LPS.
