Key technologies in synthetic biology of natural products.

Xue-jun Kuang; Li-qiu Zou; Ying LI; Chao Sun; Shi-lin Chen

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Key technologies in synthetic biology of natural products.

Author: Xue-Jun KUANG ¹ ; Li-Qiu ZOU ¹ ; Ying LI ¹ ; Chao SUN ¹ ; Shi-Lin CHEN ²
Author Information

1. Institute of Medicinal Plant Development, China Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Publication Type:Journal Article
Keywords: DNA assembly; genome editing; natural products; synthetic biology
From: China Journal of Chinese Materia Medica 2016;41(22):4112-4118
CountryChina
Language:Chinese
Abstract: Natural products with complex and diverse structures are the major sources of new drugs. The biosynthesis of natural products is considered to be one of the best ways to solve the problems of complex and scarce natural products. DNA assembly technology and genome editing technology are two key technologies in the emerging interdisciplinary field of synthetic biology. A number of novel DNA assembly methods developed in the last few years have paved the way for the engineering of high molecular weight DNA molecules, including whole genomes, hence, it can realize the reconstruction of the metabolic pathways and speed up optimization process. A wide variety of new tools for microbial genome editing will be applied widely to modify the chassis genome to increase its adaptation with the exogenetic pathways. This article summarized the latest advance with respect to DNA assembly and genome editing, which aims to provide help for reconstruction and optimization of the synthetic biological systems of natural products.