Influence of different drying methods on steroidal saponins and homoisoflavonoids in Ophiopogon japonicus.
- Author:
Fei WANG
1
;
Zhan-Peng SHANG
1
;
Zhi-Guo MA
2
;
Si-Yi LIU
1
;
Zi-Jian WANG
2
;
Jia-Yu ZHANG
3
;
Jian-Qiu LU
4
Author Information
- Publication Type:Journal Article
- Keywords: HPLC-DAD-ELSD-MSn; Ophiopogon japonicus; drying methods; homoisoflavonoids; steroidal saponins
- From: China Journal of Chinese Materia Medica 2016;41(23):4393-4399
- CountryChina
- Language:Chinese
- Abstract: To study the influence of three different drying methods (including 50 ℃-drying, 80 ℃-drying and －70 ℃-freeze-drying methods) on steroidal saponins and homoisoflavonoids in Ophiopogon japonicus,a HPLC-DAD-ELSD-MSn method was investigated to screen and identify the differential components. Through comparing the HPLC chromatograms with that of fresh O. japonicus, 50 ℃-drying medicine was similar with fresh medicine whereas the other two drying methods had great influence on the components of O. japonicus. In this study, 36 differential components were screened, among which 24 constituents(13 homoisoflavonoids and 11 steroidal saponins) were identified via HPLC-LTQ-Orbitrap MS.As a result, it was revealed that different drying methods had significant influences on the components of steroidal saponins and homoisoflavonoids. Among them, 50 ℃-drying method was the most suitable drying approach when the stability of components, cost and practicability were considered.
