The effect of DNA methyltransferase 1 low expression on the global genome DNA methylation status of 16HBE cell.
- Author:
Lin-qing YANG
1
;
Qing-cheng LIU
;
Chun-mei GONG
;
Gong-hua TAO
;
Jian-jun LIU
;
Gong-hua HU
;
Hai-yan HUANG
;
Kun-peng WANG
;
Zhi-xiong ZHUANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Cycle; Cell Line; DNA (Cytosine-5-)-Methyltransferase 1; DNA (Cytosine-5-)-Methyltransferases; genetics; metabolism; DNA Methylation; Down-Regulation; Epithelial Cells; metabolism; Humans; RNA Interference; RNA, Small Interfering; genetics
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2011;29(3):194-197
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct DNA methyltransferase 1 (DNMT1) low expression 16HBE cell line and observe the variation of cell cycle and global genomic DNA methylation.
METHODSThe method of Lenti-virus induced RNA interference was applied to introduce four different shRNA fragment into 16HBE cells. Flow cytometry and 5-mC immunofluorescence methods were used to observe the cell cycle and global DNA methylation status of DNMT1 low expression 16HBE cells.
RESULTSThe DNMT1 protein relative expression level of 16HBE-shDNMT1-4 cell line was down regulated about 44% (P < 0.05) compared with the control. No obvious differences of cell cycle and global genome DNA methylation status were observed between the 16HBE and 16HBE-shDNMT1.
CONCLUSIONThe DNMT1 gene low expression cell is successfully constructed, and there are no obvious changes happened on the cell cycle and global genomic DNA methylation.
