Effect on expression of vascular smooth muscle cell calcium channel in rats exposed in low temperature.
- Author:
Dong-mei YANG
1
;
Shu-hua ZHANG
;
Yu-zhi GE
;
Yun-xia WANG
;
Yan-feng LIU
;
Zhi-ting WU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood Pressure; Calcium Channels, L-Type; metabolism; Cold Temperature; adverse effects; Hypertension; Muscle, Smooth, Vascular; metabolism; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2011;29(3):224-226
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the expression of vascular smooth muscle cells calcium channel α1C subunit (LTCCα1C) in rats exposed in low temperature.
METHODSCold-induced hypertension was established and blood pressure was measured every two weeks. The mRNA expression of L type calcium channel α1C was determined by RT-PCR.
RESULTSThe blood pressure of the rats exposed to cold environment increased. The blood pressure of experimental groups [(102.8 ± 2.25) mm Hg] began to increase from the first two weeks, compared with the control group [(89.2 ± 3.73) mm Hg], there were significant difference (P < 0.05). The blood pressure of experimental groups were (114.5 ± 4.21), (121.9 ± 3.03) mm Hg respectively at 4, 6 weeks. Compared with the control group, the expression of LTCCα1C mRNA of the cold exposure group increased significantly (P < 0.01). There was a significant correlation between the expression of LTCCα1C mRNA and the blood pressure of the rats (r = 0.86, P < 0.01).
CONCLUSIONRepeated cold exposure can establish cold-induced hypertension, and the level of vascular smooth muscle cells LTCCα1C expression increase.
