Study on activities and protein and gene expression of renal H(+)-K(+)-ATPase in rats subchronic exposed to trimethyltin chloride.
- Author:
Na ZHAO
1
;
Gang SUI
;
Nan-Chun LI
;
Xin WU
;
Zi YANG
;
Guan-Chao LAI
;
Yan YANG
;
Zhong-Ning LIN
;
Xiao-Jiang TANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Female; Gene Expression; H(+)-K(+)-Exchanging ATPase; genetics; metabolism; Kidney; drug effects; metabolism; Rats; Rats, Sprague-Dawley; Toxicity Tests, Subchronic; Trimethyltin Compounds; toxicity
- From:Chinese Journal of Industrial Hygiene and Occupational Diseases 2011;29(4):299-302
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the activity, protein and gene expression of renal HK-ATPase (HKA) in rats subchronic exposed to trimethyltin chloride (TMT).
METHODSIn subchronic toxic test (14-week), 55 female SD rats (age, 6 weeks) were divided randomly into 5 groups: control, low, medium, high and super high dosage, respectively, which drank water with TMT of 0, 8.20, 32.81, 131.25 and 262.50 microg x kg(-1) x d(-1) for 14 weeks. Then serum K+ levels were measured; the activities of HK-ATPase (HKA) in kidneys were detected by the method of determinated phosphorus content; Western Blot assay and real-time PCR were used to exam the protein and mRNA expression levels of HKA in kidneys, respectively.
RESULTSThe serum K+ level in super-high dosage group was (5.6 +/- 0.4) mmol/L, which was significantly lower than that [(6.9 +/- 0.3) mmol/L] in control group (P < 0.01). The HKA enzymatic activity of kidneys in low and super high dosage groups was 4.50 +/- 1.45 and 4.55 +/- 0.72 micromolPi x mg prot(-1)h(-1), respectively, which were significantly lower than that (6.55 +/- 0.77 micromol Pi x mg prot(-1) h(-1)) in control group (P < 0.05).
CONCLUSIONWhen rats were exposed subchronic to TMT, the renal HKA activity could reduce, but the expression levels of HKA protein and mRNA did not decrease.
