Effect of phosphatidylinositol 3-kinase/serine-threonine kinase signalling pathway during the proliferation process of HL-60 cells exposed to benzoquinone.
- Author:
Yi-min LIU
1
;
Yong-sheng LI
;
Zhi WANG
;
Xu-dong LI
;
Xiao GUO
;
Chun-hui GU
Author Information
- Publication Type:Journal Article
- MeSH: Benzoquinones; toxicity; Cell Proliferation; drug effects; HL-60 Cells; Humans; Phosphatidylinositol 3-Kinase; metabolism; Protein-Serine-Threonine Kinases; metabolism; Signal Transduction
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2011;29(5):334-337
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of phosphatidylinositol 3-kinase/Serine-threonine kinase (PI3K/Akt) signal pathway on the proliferation of HL-60 cells exposed to benzoquinone (BQ).
METHODSHL60 cells were divided into 3 groups: control group (treated with PBS), BQ group (treated with 3 micromol/L BQ) and LY294002 plus BQ group (treated with 20 micromol/L LY294002 plus 3 micromol/L BQ). The cell proliferation was measured with alamar blue dye assay. Western blot assay was used to detect the expression of p-Akt and Akt proteins and flow cytometer was used to observe the cell cycle.
RESULTSThe cell proliferation rate and the cell proportion in the S, G2 phase of BQ group were 185.00% +/- 30.00%, 48.23% +/- 1.37% and 15.40% +/- 1.21%, respectively, which were significantly higher than those (100.00% +/- 0.00%, 42.47% +/- 0.45% and 5.40% +/- 0.40%) of control group (P<0.05). But the cell proportion rate (36.37% +/- 0.40%) in the G1 phase in BQ group was significantly lower than that (52.13 +/- 0.75%) in control group (P<0.05). The expression level of p-Akt protein in BQ group was significantly higher than that in control group (P<0.05). The cell proliferation rate and the cell proportion in the S, G2 phase of LY294002 plus BQ group were 82.59% +/- 15.00%, 42.03% +/- 0.50% and 3.87% +/- 0.47%, respectively, which were significantly lower than those of BQ group (P<0.05). But the cell proportion rate (54.43% +/- 0.40%) in the G1 phase in LY294002 plus BQ group was significantly higher than that in BQ group (P<0.05).
CONCLUSIONThe PI3K/Akt signal pathway may play an important role in the proliferation of HL-60 cells exposed to BQ.