Effects of Xue-Bi-Jing combined with forsythia suspension on the liver gene expression levels of rats with sepsis model.
- Author:
Dong-Qiang WANG
1
;
Yong-Chao TIAN
;
Shu-He ZHANG
;
Zhao-Jian ZHANG
;
Jing LI
;
Zhi-Jun LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Disease Models, Animal; Drugs, Chinese Herbal; pharmacology; Forsythia; Gene Expression; Gene Expression Regulation; Liver; metabolism; Male; Oligonucleotide Array Sequence Analysis; Phytotherapy; Rats; Rats, Wistar; Sepsis; drug therapy; genetics; metabolism
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2011;29(6):417-420
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of "XUE BI JING plus LIANQIAO" injection on gene expression levels of rats with sepsis model.
METHODSOne hundred and twenty rats were randomly divided into sham operation group, sepsis model group, Te-neng group and "XUE BI JING plus forsythia suspension" group. The sepsis model of rats was prepared by "CLP" method. Tai neng group was treated by peritoneal injection Imipenem/ Cilastatin (0.18 g/kg); "XUE BI JING plus LIANQIAO" group was treated by peritoneal injection Imipenem/ Cilastatin (0.18 g/kg) plus "xue-bi-jing" (10 ml/kg) and "liang ge san" (18 g/200 g) by intragastric administration 2 times a day; the sham operation group and model group were treated by peritoneal injection of normal saline (10 ml/kg). The survival rates at 48h and 72h were observed for all groups. The gene expression levels of livers in all groups were detected by BiostarR-40s chip. The NCBI database was used to inquest Gene function and class.
RESULTSThe survival rates at 48h and 72h in "XUE BI JING+ forsythia suspension" group were 83.3% and 76.7% which were significantly higher than those (30.0% and 16.7%) in sepsis model group and those (60.0% and 33.3%) in Te-neng group (P < 0.01). Model group/control group have 305 differential expression genes with 159 up-regulation genes and 146 down-regulation genes. Tai-neng group/model group have 386 differential expression genes with 206 up-regulation genes and 180 down-regulation genes. "XUE BI JING plus forsythia suspension" group/model group have 342 differential expression genes with 102 up-regulation genes and 240 downregulation genes. The genes with up-regulation in model group/ control group and with down-regulation in"XUE BI JING plus forsythia suspension" group/model group were 24. The genes with down-regulation in the model group/ sham operation group and with up-regulation in "XUE BI JING plus forsythia suspension"group/model group were 16.
CONCLUSION"XUE BI JING plus forsythia suspension" can reduce the mortality of rats with sepsis, which could be due to the expression of relative regulation genes.
