Study of Trichostation A-Induced Expression of Costimulatory Molecules CD80 and CD86 in Acute Myelocytic Leukemia Cells.

Mei-xia YU; Xun Liu; You-fa Chen; Yang Zhang; Jing Cheng; Dong-xia HU; Ling Zhang; Lei Feng; Xiao-li Shen; Jian NI; Yong-ming Zhou

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Study of Trichostation A-Induced Expression of Costimulatory Molecules CD80 and CD86 in Acute Myelocytic Leukemia Cells.

Author: Mei-Xia YU ¹ ; Xun LIU ² ; You-Fa CHEN ¹ ; Yang ZHANG ³ ; Jing CHENG ⁴ ; Dong-Xia HU ¹ ; Ling ZHANG ¹ ; Lei FENG ¹ ; Xiao-Li SHEN ¹ ; Jian NI ⁵ ; Yong-Ming ZHOU ⁶
Author Information

1. Department of Hematology, The Affiliated Tianyou Hospital, Wuhan University of Science and Technology, Wuhan 430064, Hubei Province, China.
2. Division of Nephrology, Department of Internal Medicine, The Third Affiliated Hospital of SUN Yat-Sen University, Guangzhou 510630, Guangdong Province, China.
3. Department of Biopharmacy, School of Pharmacy, Jilin University, Changchun 130021, Jilin Province, China.
4. Central Laboratory, The Affiliated Tianyou Hospital, Wuhan University of Science and Technology, Wuhan 430064, Hubei Province, China.
5. Department of Oncology Clinical Pharmacy, Brigham and Women's Hospital, Harvard Medical School, Boston, USA, MA 02115. E-mail: Jni2@partners.org.
6. Department of Hematology, The Affiliated Tianyou Hospital, Wuhan University of Science and Technology, Wuhan 430064, Hubei Province, China. E-mail: zhym112@126.com.
Publication Type:Journal Article
MeSH: B7-1 Antigen; B7-2 Antigen; Cell Line, Tumor; Cell Survival; Flow Cytometry; Humans; Hydroxamic Acids; Leukemia, Myeloid, Acute
From: Journal of Experimental Hematology 2015;23(6):1564-1569
CountryChina
Language:English
Abstract:
OBJECTIVETo investigate the trichostain A (TSA)-induced expression of costinmulatory molecules CD80 and CD86 in HL-60, K562 and mononuclear cells (MNC) of bone marrow in AML patients and its clinical significance.
METHODSThe TSA-induced expression of costimulatory molecules CD80, CD86 in HL-60, K562 and BMMNC, and the cell viability were detected by flow cytometry; the mRNA expression of CD80 and CD86 was detected by RT-PCR; after the TSA-induced HL-60 cells and K562 cells were irradiated with 75 Gy, the effect of these cells on proliferation of PBMNC from healthy volunteers was determined with CCK-8 method.
RESULTSThe HL-60 cells and BMMNC in AML patients expressed CD86, not expressed CD80, while the K562 cells not expressed CD86 and CD80. TSA could up-regulate the expression of CD86 in HL-60 cells and BMMNC of AML patients. The TSA-induced HL-60 cells expressing costimulatory molecule CD86 showed the proliferative effect on BMMNC from healthy volunteers.
CONCLUSIONThe TSA can induce the expression of costimulatory molecule CD86 in HL-60 cells and BMMNC in AML patients, and can improve the proliferation of PBMNC in healthy volunteers.