Study of Trichostation A-Induced Expression of Costimulatory Molecules CD80 and CD86 in Acute Myelocytic Leukemia Cells.
- Author:
Mei-Xia YU
1
;
Xun LIU
2
;
You-Fa CHEN
1
;
Yang ZHANG
3
;
Jing CHENG
4
;
Dong-Xia HU
1
;
Ling ZHANG
1
;
Lei FENG
1
;
Xiao-Li SHEN
1
;
Jian NI
5
;
Yong-Ming ZHOU
6
Author Information
- Publication Type:Journal Article
- MeSH: B7-1 Antigen; B7-2 Antigen; Cell Line, Tumor; Cell Survival; Flow Cytometry; Humans; Hydroxamic Acids; Leukemia, Myeloid, Acute
- From: Journal of Experimental Hematology 2015;23(6):1564-1569
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the trichostain A (TSA)-induced expression of costinmulatory molecules CD80 and CD86 in HL-60, K562 and mononuclear cells (MNC) of bone marrow in AML patients and its clinical significance.
METHODSThe TSA-induced expression of costimulatory molecules CD80, CD86 in HL-60, K562 and BMMNC, and the cell viability were detected by flow cytometry; the mRNA expression of CD80 and CD86 was detected by RT-PCR; after the TSA-induced HL-60 cells and K562 cells were irradiated with 75 Gy, the effect of these cells on proliferation of PBMNC from healthy volunteers was determined with CCK-8 method.
RESULTSThe HL-60 cells and BMMNC in AML patients expressed CD86, not expressed CD80, while the K562 cells not expressed CD86 and CD80. TSA could up-regulate the expression of CD86 in HL-60 cells and BMMNC of AML patients. The TSA-induced HL-60 cells expressing costimulatory molecule CD86 showed the proliferative effect on BMMNC from healthy volunteers.
CONCLUSIONThe TSA can induce the expression of costimulatory molecule CD86 in HL-60 cells and BMMNC in AML patients, and can improve the proliferation of PBMNC in healthy volunteers.