- Author:
Wen-Ming WANG
1
;
Jing WANG
1
;
Hong-Mei JING
2
Author Information
- Publication Type:Journal Article
- MeSH: Cell Proliferation; DNA Methylation; Gene Silencing; Humans; Jurkat Cells; Lymphoma, Non-Hodgkin; Protein Tyrosine Phosphatase, Non-Receptor Type 13
- From: Journal of Experimental Hematology 2015;23(6):1596-1600
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the methylation status of PTPL1 in Hut78, Maver, Z138, CA46, Raji and Jurkat cell lines, and the reversing effect of 5-Aza on expression of PTPL1 mRNA.
METHODSThe Hut78, Maver, Z138, CA46, Raji and Jurkat cell lines were cultured in vitro, the MS-PCR was used to detect the methylation status of PTPL1 gene and RT-PCR was used to detect the expression of PTPL1 mRNA, the CCK-8 method was used to determine the cell proliferation, the siRNA-mediated silencing of PTPL1 was used to clarify the role of PTPL1 in lymphoma cell lines.
RESULTSPTPL1 gene promoter was non-methylated in Hut78, Maver and Z138 cells, and was hyper-methylated in Raji, CA46 and Jurkat cells; the different levels of PTPL1 mRNA expression were in Hut78, Maver and Z138; the re-expression of PTPL1 mRNA resulted from hyper-methylation was found in Raji, CA46 and Jurkat cells. The 5-Aza treatment could inhibit the prolifenation of Raji, CA46 and Jurkat cell lines and induce re-expression of PTPL1 mRNA. After PTPL1 was interfered by siRNA, the growth of lymphoma cells was promoted.
CONCLUSIONThe PTPL1 gene silencing induced by hyper-methylation may be an important factor in the occurrence and development of non-Hodgkin lymphoma. The methylation of PTPL1 gene may be used as a possible molecular marker for diagnosis and treatment targets.