Magnetic Nanoparticles of Fe3O4 Enhance Artesunate-inducing Apoptosis of SKM-1 Cells.

Ying WANG; Yu-Xiang HAN; Shu-Kai QIAO; Zhi-Yun NIU; Ling PAN

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Magnetic Nanoparticles of Fe3O4 Enhance Artesunate-inducing Apoptosis of SKM-1 Cells.

Author: Ying WANG ¹ ; Yu-Xiang HAN ² ; Shu-Kai QIAO ³ ; Zhi-Yun NIU ³ ; Ling PAN ⁴
Author Information

1. Department of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China. E-mail: wangying730@126.com.
2. Department of Immunology and Rheumatology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
3. Department of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
4. Department of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China. E-mail: lingpan20002000@yahoo.com.
Publication Type:Journal Article
MeSH: Apoptosis; Artemisinins; Caspase 3; Cell Line, Tumor; Down-Regulation; Ferric Compounds; Humans; Inhibitor of Apoptosis Proteins; Magnetite Nanoparticles; Up-Regulation
From: Journal of Experimental Hematology 2015;23(6):1647-1651
CountryChina
Language:English
Abstract:
OBJECTIVETo investigate the inhibitory effect of the copolymer of magnetic nanoparticles of Fe(3)O(4) (MNPs-Fe(3)O(4)) and artesunate (ART) on myelodysplastic syndromes (MDS) cell line SKM-1 cells and the potential mechanisms.
METHODSThe protein expression levels of BCL-2, BAX, Caspase-3, and Survivin in SKM-1 cells treated with or without the co-polymer were measured by Western blot. The co-polymer-induced apoptosis rate of SKM-1 cells was measured by flow cytometry.
RESULTSThe apoptosis rate of SKM-1 cells in the copolymer groups was higher than that in both MNPs-Fe(3)O(4) and artesunate groups alone. The MNPs-Fe(3)O(4) may enhance ART-induced cell apoptosis. Western blot assay showed that the expression of survivin and BCL-2 protein were down-regulated in the ART group, and this down-regulation was even more significant in the group of copolymer of ART with MNPs-Fe(3)O(4). The levels of BAX were increased both in ART group and the copolymer of ART with MNPs-Fe(3)O(4) group, as compared with control group and MNPs-Fe(3)O(4) group. The levels of active-caspase-3 were obviously up-regulated when the ART was combined with the MNPs-Fe(3)O(4). The copolymer of ART with MNPs-Fe(3)O(4) could trigger changes in the expression levels of apoptosis-related genes in SKM-1 cells, among which up-regulation of BAX and down-regulation of survivin and BCL-2 are the 2 major alterations.
CONCLUSIONArtesunate can induce the apoptosis of SKM-1 cells, and MNPs-Fe(3)O(4) may enhance the cell apoptosis induced by ART.