Identification of Bx02 Alleles and Genetic Analysis of the Involved Ancestry.
- Author:
Guo-Liang LIU
1
;
Fang ZHU
1
;
Xiao-Li ZHENG
1
;
Cheng-Hua CAO
1
;
Ya-Jing HE
1
;
Xiang-Shu CHENG
1
;
Tao LI
1
;
Xin-Ying JI
2
Author Information
- Publication Type:Journal Article
- MeSH: ABO Blood-Group System; Alleles; Base Sequence; Blood Grouping and Crossmatching; Exons; Genetic Testing; Genotype; Humans; Mutation; Phenotype; Polymerase Chain Reaction
- From: Journal of Experimental Hematology 2015;23(6):1749-1752
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify the genotypes of the blood sample whose blood grouping showed discrepancies and study the ABO alleles' molecular characteristics of the involved ancestry.
METHODSBlood samples were preliminary genotyped by PCR-SSP. Complete exon 6 and 7 in the ABO genes were amplified by PCR and the PCR products were directly sequenced and cloning sequenced to identify its genotype.
RESULTSSequence analysis indicated that 3 samples of the family had an nt905A>G mutation in the B gene compared with ABO*B101. Combined with the serological results, the propositus could be typed as Bx02/O102.
CONCLUSIONDNA sequencing analysis is able to identify the serological phenotype samples that forward and reverse group methods were incongruous.
