Effect of Rapamycin on Expression of Survivin and Caspase-3 and Its Influence on K562 Cell Ultrastructure.
- Author:
Xiao-Yan ZHANG
1
;
Lin YANG
1
;
Zi-Yuan NIE
1
;
Yin-Tao SHAN
1
;
Yu-Xia PAN
1
;
Jian-Min LUO
2
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Caspase 3; metabolism; Cell Proliferation; Humans; Inhibitor of Apoptosis Proteins; metabolism; K562 Cells; drug effects; ultrastructure; RNA, Messenger; Sirolimus; pharmacology
- From: Journal of Experimental Hematology 2016;24(1):52-55
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of rapamycin on the expression of survivin and caspase-3 at mRNA level in K562 cells and the influence of rapamycin on K562 cell ultrastructure.
METHODSThe effects of rapamycin at various concentration on K562 cell proliferation were analyzed by CCK8; the morphological characteristics of K562 cells was observed by transmission electron microscopy; the expression of survivin and caspase-3 at mRNA level in K562 cells treated with rapamycin was detected by RT-PCR.
RESULTSThe proliferation of K562 cells was significantly inhibited by rapamycin. The apoptosis level of K562 cells increased with increase of rapamycin concentration, the expression of survivin at mRNA level decreased with increase of rapamycin concentration (P < 0.05). The expression of caspase-3 at mRNA level increased with increase of rapamycin concentration.
CONCLUSIONRapamycin can prornote K562 cell apoptosis through up-regulating caspase-3 level and reduceing survivin level.
