Significance of Morphological Examination, Cytochemical Staining Combined with Bone Marrow Biopsy in Differential Diagnosis of Myelodysplastic Syndrome with Low Blasts and Hemolytic Anemia.
- Author:
Li-Lin GU
1
;
Hui-Yuan KANG
1
;
Yu-Ling PAN
1
;
Gai-Xia LIU
1
;
Su-Jun GE
1
;
Mian-Yang LI
2
;
Cheng-Bin WANG
3
Author Information
- Publication Type:Journal Article
- MeSH: Anemia, Hemolytic; complications; diagnosis; Biopsy; Bone Marrow Cells; cytology; Diagnosis, Differential; Erythroid Precursor Cells; cytology; Humans; Megakaryocytes; cytology; Myelodysplastic Syndromes; complications; diagnosis; Retrospective Studies; Staining and Labeling
- From: Journal of Experimental Hematology 2016;24(1):138-143
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the value of morphological examination, cytochemical staining combined with bone marrow biopsy in the differential diagnosis between myelodysplastic syndrome (MDS) with low blasts and hemolytic anemia (HA).
METHODSThe clinical data of 85 cases of myelodysplastic syndrome with low blasts (< 5%) and 61 patients with hemolytic anemia in Chinese PLA's Gerneral hospital from September 2009 to March 2015 were retrospectively analysed. The clinical characteristics, cytogenetic and molecular features, bone marrow cell count and morphology features, cytochemical staining results and bone marrow biopsy features of above-methioned patients were compared.
RESULTSThere was no significant difference (P > 0.05) in clinical data between MDS group and HA group. Megakaryocytic dysplasia-positive rate, and ring sideroblasts positive rate, and PAS positive rate were significantly higher in MDS group than those that in HA group (P < 0.05). Abnormal localization of immature precursors (ALIP) and megakaryocytic dysplasia positive rate in bone marrow biopsy were significantly higher in MDS group than those that in HA group (P < 0.05), 90.6% of MDS with low blasts patients were identifiable by combined detections.
CONCLUSIONCombining detection of morphology, cytochemistry staining and bone marrow biopsy has been confirmed to be more useful for differential diagnosis between MDS with low blasts and HA.
