Inducing Effect of Modified Cytokine Cocktail on Dendritic Cells.

Wei XU; Bao-long Wang; Qiong Huang; Zhi-feng Zhou; Peng Luo

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Inducing Effect of Modified Cytokine Cocktail on Dendritic Cells.

Author: Wei XU ¹ ; Bao-Long WANG ² ; Qiong HUANG ³ ; Zhi-Feng ZHOU ⁴ ; Peng LUO ⁵
Author Information

1. Department of Blood Transfusion, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China.
2. Department of Clinical Laboratorial Examination, The Affiliated Provincial Hospital of Anhui Medical University, Hefei 230001, Anhui Province, China. E-mail: wbl19655@163.com.
3. Department of Clinical Laboratorial Examination, The Affiliated Anqing Hospital of Anhui Medical University, Anqing 246003, Anhui province, China.
4. Department of Clinical Laboratorial Examination, The Affiliated Hefei Hospital of Anhui Medical University, Hefei 230011, Anhui province, China.
5. Department of Clinical Laboratorial Examination, The Affiliated Provincial Hospital of Anhui Medical University, Hefei 230001, Anhui Province, China.
Publication Type:Journal Article
MeSH: Antigens, CD; metabolism; Cell Culture Techniques; Cell Differentiation; Chemokines; metabolism; Cytokines; pharmacology; Dendritic Cells; cytology; drug effects; Flow Cytometry; Granulocyte-Macrophage Colony-Stimulating Factor; pharmacology; Humans; Interleukin-4; pharmacology; Receptors, Chemokine; metabolism
From: Journal of Experimental Hematology 2016;24(1):197-204
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the inducing effect of 'modified' cytokine cocktail on the dendritic cell maturation and migration capability.
METHODSPBMNC were isolated from human peripheral blood stem cell (PBSC) by using density gradient centrifugation, the immature DC (imDC) were induced by using GM-CSF and IL-4 in vitro. Total A549 RNA was transfected into imDC by using electroporation, which was stimulated to matuation by the "gold standard" cytokine cocktail and "modified" cytokine cocktail, respectively. The expression of DC surface markers (CD11c, HLA-DR, CD80, CD83 and CD86) and chemokine receptor (CCR5, CCR7 and CXCR4) were detected by flow cytometry; the mRNA expression levels of DC chemokine receptor (CCR2, CCR5, CCR7, CXCR3 and CXCR4) and chemokine (CCL2, CCL3, CCL5, CCL19, CCL21, CXCL10 and CXCL12) were detected by RT-PCR.
RESULTSAs compared with "gold standard cytokine cocktail", the "modified" cytokine cocktail-induced DC expressed higher levels of surface markers (CD11c, HLA-DR, CD80, CD83 and CD86), chemokine receptors (CXCR4) and chemokine (CCL2, CCL3, CCL5, CCL19, CCL21, CXCL10 and CXCL12).
CONCLUSIONThe "modified" cytokine cocktail can more effectively induce the DC maturation, enhace the migratory capability of DC and more generate the immunostimulatory DC, when compared with the "gold standard" cytokine cocktail effect.