Effect of cyclosporine on regulatory T cells and Foxp3 in the peripheral blood of children with chronic aplastic anemia.
- Author:
Xi-Ge WANG
1
;
Meng WANG
;
Song LIU
;
Xiao-Ge WANG
;
Jun-Ying QIAO
;
Yi-Ming CAO
;
Yu-Jie ZHOU
;
Juan YANG
;
Xiao-Ming ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Anemia, Aplastic; drug therapy; immunology; Child; Child, Preschool; Chronic Disease; Cyclosporine; pharmacology; therapeutic use; Female; Forkhead Transcription Factors; blood; genetics; Humans; Immunosuppressive Agents; pharmacology; Male; RNA, Messenger; blood; T-Lymphocytes, Regulatory; drug effects
- From: Chinese Journal of Contemporary Pediatrics 2011;13(12):936-939
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the expression diversification of CD4(+)CD25(+)CD127(low) regulatory T (Treg) cells and Foxp3 mRNA in the peripheral blood of children with aplastic anemia after the treatment with cyclosporine.
METHODSFifty children with chronic aplastic anemia were enrolled, among whom 30 received cyclosporine treatment (cyclosporine group) and 20 were treated with conventional methods (conventional group). Twenty healthy children were enrolled as the control group. The expression of CD4(+)CD25(+)CD127(low) Treg cells was detected by flow cytometry. The expression of Foxp3 mRNA was detected by real-time Q-PCR.
RESULTSThe expressions of Foxp3 mRNA and CD4(+)CD25(+)CD127(low)Treg cells showed no significant difference between the cyclosporine and the control groups 6 months after treatment. On the contrary, there were significantly lower expressions of both in the conventional group than in the control group (P<0.05). Meanwhile, the cyclosporine group had significantly higher expressions of Foxp3 mRNA and CD4(+)CD25(+)CD127(low) Treg cells than the conventional group (P<0.05).
CONCLUSIONSThe expressions of CD4(+)CD25(+)CD127(low) Treg cells and Foxp3 mRNA in children with aplastic anemia increase after cyclosporine treatment.
