Gene expression of Notch1 and Jagged1 in children with acute leukemia.
- Author:
Xiao-Li ZHAO
1
;
Kai-Li PAN
;
Xin-Hong QIAN
;
Ying-Xia LI
;
Li DU
;
Ying-Juan WANG
;
Jian-Feng LUO
;
Yao ZHANG
;
Huan QIANG
Author Information
- Publication Type:Journal Article
- MeSH: Acute Disease; Adolescent; Calcium-Binding Proteins; genetics; Child; Child, Preschool; Female; Gene Expression Regulation, Neoplastic; Humans; Infant; Intercellular Signaling Peptides and Proteins; genetics; Jagged-1 Protein; Leukemia; metabolism; Leukemia, Myeloid, Acute; metabolism; Leukemia-Lymphoma, Adult T-Cell; metabolism; Male; Membrane Proteins; genetics; Receptor, Notch1; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Serrate-Jagged Proteins; Signal Transduction
- From: Chinese Journal of Contemporary Pediatrics 2012;14(2):105-109
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the gene expression of Notch1 and Jagged1 in children with acute leukemia (AL) and their possible roles in the pathogenesis of AL.
METHODSMononuclear cells from bone marrow or peripheral blood of 47 children with AL and 20 controls (normal children or children with nonmalignant hematologic disease) were collected from February 2009 to July 2011. A two-step method to semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the gene expression of Notch1 and Jagged1. Of the 47 children with AL, there were 26 cases of B-ALL, 6 cases of T-ALL and 15 cases of AML.
RESULTSThe positive expression rate of Notch1 in the ALL and AML groups was higher than in the control group (P<0.05). The expression level of Notch1 in T-ALL children was higher than in B-ALL children (P<0.01). The positive expression rate of Jagged1 in the ALL and AML groups was not significantly different from the control group, however, the expression level of Jagged1 in the ALL and AML groups was higher than in the control group (P<0.05).
CONCLUSIONSThere are significant differences in the gene expression of Notch1 between children with different types of ALL, and a higher expression of Notch1 relates to T-ALL. The activation of Notch1 signal is common in children with AL. The abnormal gene expression of Notch1 in children with AML shows the role of Notch1 in AML. The gene expression of Jagged1 in children with ALL or AML is abnormal, and this needs to be confirmed by further research.
