Effect of NF-κB activation on the radiation response of esophageal cancer cells.
- Author:
Baozhong LI
1
;
Zhaoli CHEN
1
;
Fang ZHOU
1
;
Jie HE
2
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Western; Cell Line, Tumor; Cell Nucleus; Esophageal Neoplasms; metabolism; radiotherapy; Humans; Immunohistochemistry; NF-kappa B; metabolism; Radiation Tolerance; Signal Transduction
- From: Chinese Journal of Oncology 2014;36(7):485-488
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of NF-κB activation on radiation response of esophageal carcinoma.
METHODSThe expression of NF-κB was detected in pretreatment and posttreatment specimens of patients with ESCC by immunohistochemistry. Electrophoretic mobility shift assay (EMSA) and Western blot were used to detect the activation of NF-κB in esophageal cancer cell line KYSE150 cells. SN50, a specific NF-κB inhibitor, was applied to inhibit the activation of NF-κB. Clone formation test was used to detect the radiosensitivity of esophageal cancer cells.
RESULTSThe median survival time of patients with activated and inactivated NF-κB in the pretreatment specimens were 16 and 19 months, respectively, with a non-significant difference between the two groups (P > 0.05). As to the patients with activated and inactivated NF-κB in posttreatment specimens, the median survival times were 13 and 35 months, respectively, with a significant difference (P < 0.01) between them. Western blot showed that the cytoplasmic expression of NF-κB was reduced with increasing radiation dose at 1.5 and 3 hours after radiation treatment. However, the expression of NF-κB in the cell nuclei was increased under the same condition, showing a trend of increased nucleus/cytoplasm ratio. The clone number in SN50 group was 96.66, 64.66, 76.66 and 10.00 under 0, 2, 4 and 12 Gy irradiation, which demonstrated a significant difference compared with the control groups (P < 0.001).
CONCLUSIONSOur results show that activation of NF-κB is induced by radiotherapy. Activation of NF-κB reduces the outcome of radiation treatment of esophageal cancer patients.