Detection and clinical implication of aneuploidy of chromosomes 11 and 20 in gastric adenocarcinoma.
- Author:
Wei GAO
1
;
Shangmei LIU
2
;
Haizhen LU
;
Jing LIANG
;
Mingrong WANG
;
Xin XU
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; diagnosis; genetics; Aneuploidy; Chromosomes, Human, Pair 11; Gastric Mucosa; Humans; Lymphatic Metastasis; Neoplasm Recurrence, Local; diagnosis; genetics; Stomach Neoplasms; diagnosis; genetics
- From: Chinese Journal of Oncology 2014;36(10):761-765
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe aim of this study was to find out the value of chromosome aneuploidy in early diagnosis and prediction of postoperative recurrence of gastric adenocarcinoma (GAC).
METHODSTissue samples, including 49 GAC, pairing pericancerous mucosa and normal gastric mucosa from the distant cutting margin were use in this study. Two centromere probes, Cen11 and Cen20 specific for chromosomes 11 and 20 were analyzed by FISH . The clinicopathological data were summarized.
RESULTSThe incidence of aneuploidy of chromosome 11 in the tumors, pericancerous mucosa and normal gastric mucosa from the distant cutting margin were 83.7%, 40.8%, and 16.3%, respectively (P < 0.001), and those of chromosome 20 were 87.8%, 53.1%, and 16.3%, respectively (P < 0.001). The aneuploidy of Cen 11 displayed a significant correlation with Lauren's claasification, and lymph node metastasis (P < 0.05 for both). The pericancerous mucosa and normal gastric mucosa from the distant cutting margin displayed mainly chronic inflammatory changes, intestinal metaplasia and dysplasia.
CONCLUSIONSAneuploidy of Cen11 and Cen20 in pericancerous mucosa may be used as a candidate marker for early diagnosis of gastric adenocarcinoma and may have a predictive value of postoperative recurrence.
