Effects of mixed-tocopherols and eicosapentaenoic acid on oxidized LDL-induced oxidative damage and inflammatory cytokine secretion in human umbilical vein endothelial cells
10.3760/cma.j.issn.0253-3758.2011.03.016
- VernacularTitle:复合维生素E及二十碳五烯酸对氧化型低密度脂蛋白刺激的人脐静脉内皮细胞氧化损伤及炎症因子的影响
- Author:
Ming-Bin XIE
1
;
Mei-Lin LIU
;
Ying-Shuo HUANG
;
Pei LI
;
Ya-Bing YANG
;
Xia-Huan CHEN
Author Information
1. 杭州市红十字会医院
- Keywords:
Vitamin E;
Eicosapentaenoic acid;
Antioxidants;
Interleukin-6
- From:
Chinese Journal of Cardiology
2011;39(3):242-246
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of either alone or combined mixed-tocopherols combined with eicosapentaenoic acid (EPA) and α-Tocopherol use on oxidized LDL (oxLDL) induced 8-hydroxy-2'-deoxyguanosine (8-OHDG) and interleukin-6 (IL-6) secretion by human umbilical vein endothelial cells(HUVECs) and to explore the potential mechanism. Method Cultured HUVECs in vitro were incubated with oxLDL, oxLDL + α-tocopherol, oxLDL + mixed-tocopherols, oxLDL + EPA, oxLDL + α-tocopherol + EPA, oxLDL + mixed-tocopherols + EPA for 24 hours, respectively. Secretion of 8-OHDG and IL-6 were detected by cell enzyme linked immunosorbent assay (ELISA). The expressions of superoxide dismutase (SOD), protein kinase C-δ (PKC-δ), phosphorylated PKC-δ (p-PKC-δ) were analyzed by Western blot. Results 8-OHDG and IL-6 secretion of HUVECs was significantly increased significantly after incubated with oxLDL for 24 hours which could be significantly attenuated in the presence of tocopherols and EPA ( alone or in combination, all P ＜ 0. 05 ) while the strongest inhibition effects were seen with combined use of mixed-tocopherols and EPA. Moreover, combination of mixed-tocopherols and EPA could also significantly increase SOD activity and decrease PKC activity (all P ＜ 0. 05 ). However, the protein expression of SOD and PKC-was similar among groups. Conclusion Combined mixed-tocopherols + EPA use enhanced the inhibiting effects on the secretion of 8-OHDG and IL-6 in oxLDL stimulated HUVECs which might be linked with increased SOD activity and reduced p-PKC activity.
