MicroRNA-144 over-expression induced myocytes apoptosis.
- Author:
Fang HUANG
1
;
Xiao-yan HUANG
;
Dong-sheng YAN
;
Xi ZHOU
;
De-ye YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; genetics; Caspase 3; metabolism; Cell Line; MicroRNAs; genetics; metabolism; Muscle Cells; metabolism; Rats; Sincalide; metabolism; Transfection
- From: Chinese Journal of Cardiology 2011;39(4):353-357
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of microRNA-144 (miR-144) expression on H9C2 (2-1) myocytes.
METHODSMiR-144 was up-regulated in primary cultured H9C2 (2-1) myocytes through transfection. Cells transfected with Lipofectamine(TM) 2000 and its mixture with miRNA synthesized randomly as blank control and negative control respectively. The up-regulation of miR-144 was confirmed by real-time PCR. Cell apoptosis was evaluated by means of CCK-8, Caspase-3 and flow cytometry.
RESULTSReal-time PCR results showed that the miR-144 expression was obviously increased in miR-144 up-regulation group (2178.84 ± 838.52) compared with negative (2.06 ± 0.73) and blank (1.00 ± 0.00) control group (all P < 0.01). The proliferation was lower, the activity of Caspase-3 was elevated and the apoptosis rates were significantly increased in miR-144 up-regulation group compared with negative and blank control group, while no significant difference was found between the latter 2 groups.
CONCLUSIONMiR-144 mimics may selectively up-regulate the expression of miR-144 in myocardial cells and consequently promote apoptosis and inhibit proliferation in myocardial cells.
