Effect of valsartan and carnitine on cardiomyocyte Calpain-1 and Bcl-xl expressions of dogs with chronic alcohol intake-induced cardiomyopathy
10.3760/cma.j.issn.0253-3758.2011.05.013
- VernacularTitle:钙激活中性蛋白酶Calpain-1在犬酒精性心肌病心肌细胞凋亡中的表达及不同药物对其的干预作用
- Author:
Ying SANG
1
;
Li-Jun ZHOU
;
Ling JING
;
Lin YUAN
;
Li-Xin LU
;
Qing-Hui ZHANG
Author Information
1. 尚志市人民医院
- Keywords:
Cardiomyopathy,alcoholic;
Calpain;
Carnitine;
Apoptosis;
Valsartan
- From:
Chinese Journal of Cardiology
2011;39(5):440-445
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of valsartan and carnitine on cardiomyocyte Calpain-1and Bcl-xl expressions of dogs with chronic alcohol intake-induced cardiomyopathy.Methods Dogs were randomly assigned into 4 groups(n=7 each):(1)alcohol fed(free access to 5%,1st week;10%2nd week;500 ml 25%bolus plus free access to 5%from 3 to 24 weeks,A);(2)alcohol+valsartan (5 mg·kg-1·d-1,B);(3)alcohol+carnitine(300 mg·kg-1·d-1,C);(4)Control(D).After six months,all animals were assessed for left ventricular(LV)function by echocardiography.The Bad and Bcl-xl protein expressions were evaluated by immunohistochemistry.The expression of Calpain-1 protein was determined with Western blot.Myocardial morphology was quantified on HE stained slices and under electron microscopy.The terminal deoxynucleotidyl transferase-mediated iotin-dUTP nick end-labeling(TUNEL)was performed for apoptosis analysis.Results Compared with group D.LVEDD and LVESD were significantly increased while EF and FS significantly decreased in group A.In alcohol fed group,expressions of Bad and Calpain-1 protein were significantly increased while Bcl-xl protein expression was downregulated,all changes could be significantly attenuated by intervention with valsartan and carnitine(all P<0.05).Conclusion These data suggest that alcohol could promote cardiac myocyte apoptosis,reduce cardiac function and aggravate myocardial remodeling which valsartan and carnitine could reduce alcoholic cardiomyopathy by downregulating Calpain-1 and Bad protein expression and upregulating expression of Bcl-xl protein.
