Expression of Toll-like receptor 4 and tumor necrosis factor-α on peripheral-blood mononuclear cells and their correlation with myocardial perfusion in patients with diabetic cardiomyopathy
10.3760/cma.j.issn.0253-3758.2011.06.006
- VernacularTitle:糖尿病心肌病外周血单核细胞Toll样受体4、肿瘤坏死因子-α的表达及其与心肌灌注的相关性
- Author:
Keng WU
1
;
Qiong YOU
;
Hai-Liang MO
;
Rui-Na HUANG
Author Information
1. 广东医学院附属医院
- Keywords:
Diabetes mellitus;
Cardiomyopathies;
Toll-like receptor 4;
Tumor necrosis factor-alpha;
Myocardial perfusion
- From:
Chinese Journal of Cardiology
2011;39(6):503-507
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of Toll-like receptor 4(TLR4) and tumor necrosis factor-α (TNF-α) on peripheral-blood mononuclear cells(PBMCs) and their correlation with myocardial perfusion in patients with diabetic cardiomyopathy (DCM).Methods The expression of TLR4 and TNF-α mRNA on PBMCs were examined by SYBR GreenⅠreal-time quantitative reverse transcription ploymerse chain reaction (RT-PCR), the levels of TLR4 and TNF-α were examined by flow cytometric analysis and enzyme-linked immuno sorbent assay (ELISA) on DCM group (n=20) , Type 2 diabetic group (n=22) and control group (n=20). Myocardial perfusion was visualized by single-photon emission computed tomography (SPECT). Results The expressions of TLR4 and TNF-α mRNA/protein on PBMCs in DCM group were significantly higher than in Type 2 diabetic group, and higher in Type 2 diabetic group than in control groups(P<0.05); summed stress score (SSS)and summed rest score (SRS) of myocardial perfusion in DCM group were significantly higher than in Type 2 diabetic group, and higher in Type 2 diabetic group than in control groups(P<0.01).The expression of TLR4, TNF-α was positively correlated with SSS (r=0.75,P<0.05;r=0.931,P<0.005) and SRS(r=0.78,P<0.005; r=0.789,P<0.005). SSS and SRS in DCM group were also positively correlated with soluble vascular cell adhesion molecule-1 (sVCAM-1) (r=0.728,P<0.005; r=0.738,P<0.005) but there was no correlation between SSS and SRS and brain natriuretic peptide, LVEF, E/A, HbA1c, FBG, FIN and LDL-C (P>0.05).Conclusion The increased expression of TLR4 and TNF-α mRNA/protein on PBMCs and increased serum sVCAM-1 is linked with reduced myocardial perfusion in DCM group. TLR4 and TNF-α may thus play a critical role in the myocardial perfusion inflammation injury in these patients.
