Effect of valsartan on cyclooxygenase-2 expression in cultured human umbilical vein endothelial cells stimulated by ox-LDL
10.3760/cma.j.issn.0253-3758.2011.06.009
- VernacularTitle:缬沙坦对氧化低密度脂蛋白诱导的人内皮细胞环氧合酶-2表达影响的实验研究
- Author:
Li-Qing JIANG
1
;
Da-Yi HU
Author Information
1. 南方医科大学附属沧州市人民医院
- Keywords:
Atherosclerosis;
Inflammation;
Lipoprotein,LDL;
Cyclooxygenase 2;
Valsartan
- From:
Chinese Journal of Cardiology
2011;39(6):518-521
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of valsartan on cyclooxygenase-2 (COX-2) in cultured human umbilical vein endothelial cells (HUVECs) stimulated by ox-LDL.Methods HUVECs were cultured in endothelial basal medium and divided into four groups (n=5 each):group Ⅰ, control group without any treatment; groupⅡ: HUVECs stimulated with ox-LDL(100 mg/L)in endothelial basal medium for 24 hours; groupⅢ: HUVECs treated with ox-LDL (100 mg/L)and valsartan(10 μmol/L) in endothelial basal medium for 24 hours; groupⅣ:HUVECs treated with ox-LDL(100 mg/L)and valsartan(30 μmol/L) in endothelial basal medium for 24 hours. Expression of COX-1 and COX-2 mRNA were determined by reverse transcription-polymeras chain reaction(RT-PCR).Results Expression of and COX-2 mRNA was significantly higher in ox-LDL-treated HUVECs than in control group (1.478±0.104 vs. 0.366±0.104,P<0.05) ,while expression of COX-1 mRNA was similar between the 2 groups (P＞0.05). Valsartan dose-dependently decreased the COX-2 mRNA expression (group Ⅲ vs. groupⅡ:1.074±0.112 vs. 1.478±0.104,P<0.05; group Ⅳ vs. groupⅡ: 0.664±0.104 vs.1.478±0.104, P<0.05). Expression of COX-1 mRNA in ox-LDL-treated HUVECs was not affected by valsartan.Conclusions COX-2 mRNA expression in ox-LDL-treated HUVECs could be reduced by valsartan suggesting valsartan might attenuate atherosclerosis by reducing ox-LDL-induced inflammatory responses.
