Effect of the costimulatory molecules OX40-OX40 ligand interaction on the expression of NFATc1 in leukocytes of apolipoprotein E-deficient mice
10.3760/cma.j.issn.0253-3758.2011.06.011
- VernacularTitle:共刺激分子OX40-OX40L相互作用对ApoE-/-小鼠淋巴细胞活化T细胞核因子C1表达的影响
- Author:
Liang-Jie XU
1
;
Jin-Chuan YAN
;
Biao WANG
;
Pei-Jing LIU
;
Jie GONG
;
Cui-Ping WANG
Author Information
1. 江苏大学医学院附属医院
- Keywords:
Atherosclerosis;
OX40;
OX40 ligand;
NFATC transcription fators
- From:
Chinese Journal of Cardiology
2011;39(6):526-530
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of OX40/OX40L interaction on the nuclear factor of activated T cells c1(NFATc1) in ApoE-/- mice.Methods Lymphocytes were prepared from mouse spleens after Collar-treated Surgery, then incubated with a range of agonistic anti-OX40 mAbs and inhibitory anti-OX40L mAb to stimulate or inhibit OX40-OX40L interaction in vitro. The expression of NFATc1 mRNA and protein in lymphocytes of ApoE-/- mice was measured by Real Time PCR and flow cytometry, respectively.Results (1)After stimulating OX40-OX40L signal pathway, the expression of NFATc1 mRNA and protein in leukocytes of ApoE-/- mice was significantly increased, with maximal effect occurring at 20 μg/ml anti-OX40 mAb- stimulated, and peaked at 24 h at any concentration(P<0.01). (2)Anti-OX40L mAb significantly suppressed the expression of NFATc1 in leukocytes of ApoE-/- mice, with maximal effect occurring at 20 μg/ml anti-OX40L mAb, and peaked at 24 h(P<0.001).ConclusionsOX40-OX40L interaction can regulate the mRNA and protein expressions of NFATc1 in lymphocytes of ApoE-/- mice.
