Effect of microwave irradiation on neurocyte mitochondrial ultrastructure and mtTFA mRNA expression in rats cerebral cortex and hippocampus.
- Author:
Yan XIE
1
;
Hai-hong JIANG
;
Qian-fen GONG
;
Guang-bin ZHANG
;
Jian-hua YU
;
Zheng-ping YU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cerebral Cortex; metabolism; radiation effects; DNA-Binding Proteins; genetics; Hippocampus; metabolism; radiation effects; Male; Microscopy, Electron; Microwaves; adverse effects; Mitochondria; metabolism; radiation effects; ultrastructure; Mitochondrial Proteins; genetics; Nuclear Proteins; genetics; RNA; genetics; metabolism; RNA, Messenger; genetics; metabolism; Rats; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction; Transcription Factors; genetics
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2004;22(2):104-107
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore molecular controlling mechanism of mitochondrial injury induced by different density of microwave irradiation.
METHODSRats were exposed to microwave irradiation for 1 hour at average power density of 3 mW/cm(2) or 30 mW/cm(2). After microwave irradiation, the changes of pathological ultrastructure of rat cerebral cortex and hippocampus were observed by electron microscope, and mitochondrial transcription factor A (mtTFA) mRNA expression level were determined by RT-PCR.
RESULTSAfter 3 mW/cm(2) microwave irradiation for 0, 3, 24 h, mitochondrial ultrastructure and mtTFA mRNA expression level didn't significantly change in rat cerebral cortex and hippocampus. After 30 mW/cm(2) microwave irradiation for 0, 3, 24 h, mitochondrial ultrastructure obviously changed, mtTFA mRNA expression in rat hippocampus significantly increased by 67.00%, 80.00%, 30.00% respectively, and in rat cerebral cortex by 133.00%, 86.00%, 233.00% respectively. There were significant differences between the corresponding groups of hippocampus and cerebral cortex (P < 0.01).
CONCLUSIONNo obvious change in mitochondria was found after 3 mW/cm(2) microwave irradiation, but it was found after 30 mW/cm(2) microwave irradiation. Mitochondria injury in cerebral cortex was more severe than that in hippocampus. mtTFA mRNA may have certain regulation in mitochondrial energy metabolism.
