Effects of lithium on primary cultured cerebrocortical neurons of rat.
- Author:
Fang YANG
1
;
Ji-sheng LI
;
Feng YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Cell Division; drug effects; Cell Survival; drug effects; Cells, Cultured; Cerebral Cortex; cytology; Lithium; pharmacology; Neurons; cytology; drug effects; Neuroprotective Agents; pharmacology; Rats; Rats, Wistar
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2004;22(3):188-190
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the neuroprotection and the impact on brain development of lithium, the effects of lithium salt on the growth and survival of primary cultured cerebrocortical neurons were studied.
METHODSThe technique of primary cultured cerebrocortical neurons of newborn rats with serum-free medium was established, and the growth and survival of neurons treated with different doses of lithium chloride (0.625, 1.250, 2.500, 5.000, 10.000 mmol/L) were observed. The length of neuronal synapse, cell viability by MTT reduction assay were also measured.
RESULTSThe neurons were brighter, germinated rapidly, the neuronal synapse lengthened markedly, and the neurons viability was also better after treated with lithium chloride. Among the five doses, 5.000 mmol/L had the best effect [(53.80 +/- 5.84) micro m, P < 0.01].
CONCLUSIONLithium chloride can promote the growth and survival of neurons.
