OBJECTIVETo explore the effect of platelet concentration in platelet-rich plasma (PRP)' on platelet aggregation rate, to determine the allowed range of inducer adenosine diphosphate (ADP: 5.0 µmol/L, ADP: 1.5 µmol/L) and arachidonic acid (AA: 500.0 µg/ml) for platelet concentration in platelet aggregation rate.

METHODSThe venous blood samples of 72 healthy persons with citrate anticoagulation were selected and divided into groups, every 12 cases were mixed for each group. After the samples were mixed, different levels of platelet concentrations were prepared, and platelet aggregation rate of 6 groups with different platelet concentrations by ADP and AA inducer were detected by Helena platelet aggregation analyzer respectively.

RESULTSWhen the concentration of the inducer increased, the platelet aggregation rate of different platelet concentrations increased. With the platelet concentration increased, AA and ADP (15 µmol/L) induced platelet aggregation rate showed a rapid increase at first and then showed slowing, when the Plt concentration is less than 200×10/L, AA induced platelet aggregation rate decreased with the steep drop, and ADP induced platelet aggregation rate increased slightly slowly along with platelet concentration increasing. When platelet count lower than 200×10/L and AA 500 µg/ml and ADP 15 µmol/L were used for induction, the changes of platelet aggregation rate of different concentrations of platelet was slow, less volatile and more stable.

CONCLUSIONWhen the platelet aggregation rate was detected, the appropriate platelet concentration of AA and ADP inducing agents should be greater than 200×10/L.