Effect of MicroRNA-214 on Migration of Cord Blood CD34Cells Induced by Bone Marrow Mesenchymal Stem Cells.

Jing Ming; Jian Wang; Xu YE; Hui XU; Xing-bing Wang

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Effect of MicroRNA-214 on Migration of Cord Blood CD34Cells Induced by Bone Marrow Mesenchymal Stem Cells.

Author: Jing MING ¹ ; Jian WANG ¹ ; Xu YE ¹ ; Hui XU ¹ ; Xing-Bing WANG ²
Author Information

1. Department of Hematology, Anhui Provincial Hospital of Anhui Medical University, Hefei 230001, Anhui Province, China.
2. Department of Hematology, Anhui Provincial Hospital of Anhui Medical University, Hefei 230001, Anhui Province, China.E-mail: wangxingbing91@ hotmail.com.
Publication Type:Journal Article
From: Journal of Experimental Hematology 2017;25(3):661-667
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the possible effect of MicroRNA-214 (miR-214) on migration of umbilical cord blood CD34hematopoietic stem/progenitor cells induced by BM-MSC.
METHODSAfter transfection of the inhibitor or mimic of miR-214, the cultured supernatant of BM-MSC were collected respectively. The expression of miR-214 in BM-MSC was detected by real time quantitative PCR, the effect of supernatant on CD34cell migration was evaluated by chemotaxis assays. The levels of chemokine(SDF-1) secreted by BM-MSC in the supernatant were detected by ELISA.
RESULTSThe cultured supernatant of BM-MSC could promote the migration of CD34cells. Compared with the group without transfection or negative control(NC) group, the transfection with miR-214 mimic could promote the migration of CD34cells (P<0.01), while the migration rate in miR-214 inhibitor groups decreased significantly (P<0.01). Further study found that the concentration of SDF-1 was not changed notably in all groups (P>0.05), as compared with the control group.
CONCLUSIONmiR-214 signalings may indirectly increase the migration of CD34hematopoietic stem/progenitor cells by modulating BM-MSC functions, which may not significantly correlate with the chemokine SDF-1 secreted by BM-MSC.