RAPD analysis on different varieties of Rehmannia glutinosa.
- Author:
Jing-li CHENG
1
;
Lu-qi HUANG
;
Ai-juan SHAO
;
Shu-fang LIN
Author Information
- Publication Type:Journal Article
- MeSH: DNA, Plant; genetics; Plant Leaves; genetics; Plants, Medicinal; genetics; Polymorphism, Genetic; Random Amplified Polymorphic DNA Technique; Rehmannia; genetics
- From: China Journal of Chinese Materia Medica 2002;27(7):505-508
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo provide molecular evidences for its breeding by studying the genetic relationship among varieties of Rehmannia glutinosa.
METHODNineteen varieties were detected by Randomly Amplified Polymorphic DNA(RAPD) markers.
RESULTThe 20 selected primers produced 163 bands, among which 114(69.9%) were polymorphic. A DNA molecular dendrogram was established based on Hierarchical cluster analysis of 163 DNA bands amplified by 20 primers, which divided the 19 varieties into four groups: Group Beijing, Group 85-5, Group Guolimao and the other Group.
CONCLUSION8 varieties of Group Beijing have a close genetic relationship, and so have varieties of Group 85-5, which provides information for Rehmannia glutinosa's breeding.
