HPLC determination of strychnine and brucine in Semen Strychni and its processed products.
- Author:
Yi-hao JIANG
1
;
Wu-liang YANG
;
Qian-feng GONG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Hot Temperature; Plants, Medicinal; chemistry; Seeds; chemistry; Strychnine; analogs & derivatives; analysis; Strychnos nux-vomica; chemistry; Technology, Pharmaceutical; methods
- From: China Journal of Chinese Materia Medica 2002;27(12):899-901
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThis paper reports a HPLC method for determinition of strychnine and brucine in Semen Strychni and its processed products of Jiangxi method and innovated methed.
METHODSiO2 was used as the stationary phase, n-hexane-dichloromethane-methanol-ammonia(47.5:47.5:5:0.35) as the mobile phase, with detection wavelength of 254 nm.
RESULTThe contents of strychnine and brucine in the processed products of Jiangxi are lower.
CONCLUSIONThis method is accurate, simple and reliable.
