Determination of stilbene derivative in shengfa powders by HPLC.
- Author:
Ben-quan HU
1
;
Ya-jun SHI
;
Ping HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Drug Combinations; Drug Stability; Drugs, Chinese Herbal; analysis; Glycosides; analysis; Plant Roots; chemistry; Plants, Medicinal; chemistry; Polygonum; chemistry; Powders; chemistry; Quality Control; Stilbenes; analysis
- From: China Journal of Chinese Materia Medica 2002;27(12):913-948
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a new method for the determination of the idiosyncratic component, 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside in Shengfa Powders.
METHODA HPLC method was set up, using Hypersil BDS C18 column (5 microns, 4.6 mm x 150 mm), acetonitrile-water(18:82) as mobile phase, with detection at 320 nm.
RESULTThe calibration curve was linear in the range of 0.45-2.25 micrograms, r = 0.9998, the average recovery was 99.5%, RSD = 1.2%(n = 6).
CONCLUSIONThe active constituent 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside in Shengfa Powders can be separated effectively. This method is simple, specific and exact.
